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Amelioration Of Pathological Effects Of New Castle Disease By Aloe Vera

by Sayyed Raza Ali Shahid (2014-VA-515) | Prof. Dr. Zafar Iqbal Chaudhry | Dr. Gulbeena Saleem | Prof. Dr. Aftab Ahmed Anjum.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Poultry industry has many threats from the infectious diseases. Newcastle disease is the most important disease of the poultry as it is distributed all over the world and it can cause huge economic losses in poultry industry. It is caused by the Newcastle disease virus (NDV) that can infect about 240 species of birds. Newcastle disease cause immune suppression in birds. It is reported that supplementations of Aloe vera enhances the immune status and reduce inflammation. So this research project was design to observe the effect of Aloe vera on lymphoid organs, growth performance and antibody response in Newcastle disease challenge birds. For this research a total of 120 broiler chicks were divided into four groups A, B, C, and D. Group A was control group while B and C were treated with 2 percent aqueous extract of Aloe vera. Group C was also vaccinated against New Castle disease. Aloe vera was given to group B and C from day one to end of study trial. Both of the groups were challenged with ND virus at day 21. Group D was vaccinated against ND and was challenged with ND virus at day 21 without supplementation of Aloe vera. Blood samples were collected at day 1, 7, 20, 24, 26 and 28 to determine the antibody titer against ND. Highest antibody titers were observed in group C as compared to all other groups which was vaccinated against ND along with supplemented with Aloe vera. For gross pathology and histopathology, lymphoid organs were collected at day 24, 26 and 28..The average feed intake of group A and D was significantly higher than group B and C before challenge of virus but the body weight gain of 2% Aloe vera supplemented broiler was significantly (p<.05) higher than without treatment of Aloe vera. The FCR of birds supplemented with Aloe vera treatment was significantly different from the birds without Aloe vera treatment. The FCR value of group C and B was higher than A and D. lxxv A significant difference was observed in the weight of lymphoid organs of birds treated with Aloe vera as weight of organs was less in group C followed by group B, group A and group D. This was due to anti-inflammatory effects of Aloe vera. Microscopic examination revealed congestion, depletion of lymphocytes, dysplasia of thymic lobules, thinning of cortex, focal necrosis, disappearance of lymph follicles and inter-follicular edema like lesions within lymphoid organ of the groups challenged with Newcastle disease virus. However, cellular hypertrophy and decreased lymphocytes population were prominent changes in lymphoid organs of broiler treated with 2% Aloe vera. To check the virucidal effects of Aloe vera, a separate experiment was conducted in which 9 day old embryonated eggs were inoculated with ND virus along with 2 percent Aloe vera gel extract after incubation at 37Cº for an hour in group A while only ND virus was inoculated in group B. Candling was performed to see the survival of embryos in both groups which revealed a significant difference i.e. 16 percent embryos were found dead in group A while 80 percent was found dead in group B. Later on the amnioallantoic fluid of the eggs was used for spot Haemagglutination test. Group A showed less agglutination activity then group B. From this study it was concluded that Newcastle disease caused immune suppression and damage of vital organs in broiler while Aloe vera have immunomodulatory, anti-inflammatory and antiviral effects as it raised antibody titer against Newcastle disease virus and lowered the inflammatory processes along with inactivation of ND virus. It also promotes growth performance of broilers and helps the birds to survive against lethal ND disease. Availability: Items available for loan: UVAS Library [Call number: 2525-T] (1).

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Pathogenesis Of Field Isolates Of Mannheimia Hemolytica In Experimentally Infected Rabbits

by Syeda Fakhra Waheed (2014-VA-10) | Prof. Dr. Zafar Iqbal Chaudhry | Prof. Dr. Asim Aslam | Prof. Dr. Aftab Ahmed Anjum.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Shipping fever is one of the most economically important infectious diseases of ruminants with a wide prevalence throughout the continents. The disease is characterized by an acute febrile course with severe fibrinous bronchopneumonia. Infected animals may die within a few days of the onset of clinical signs, but those which survive the acute attack may become chronically infected. Both Mannheimia and Pasteurella species are commensally resident in the respiratory tract of healthy ruminants and are capable of causing infection in animals with compromised pulmonary defense system. Bovine respiratory disease (BRD) is the most common and costly problem encountered in stocker or feedlot calves. BRD also called “shipping fever”, accounts for major economic losses to the producer by reducing average daily gain, feed efficiency, and overall performance of beef calves. The aim of present study was isolation of M.haemolytica from cattle. The identification of organism was performed through biochemical tests and confirmation by polymerase chain reaction. The nature of disease was evaluated through gross and microscopic lesions. A total of 50 tissue samples (25 lungs and 25 pharynx) were collected from Punjab Agriculture and Meat company Lahore and brought to the Department of Pathology UVAS, Lahore and were analyzed for biochemical and molecular detection of M .haemolytica. For studying the pathogenesis of the disease, experimental infection was given to rabbits in Department of Pathology, UVAS Lahore. Rabbits were randomly divided into Group A, Group B and Group C with nine rabbits (n=9) in each group. Experimental infection of field isolated M. hemolytica was given intratrachealy to the rabbits. Rabbits of group A and B were infected with 0.5 mL bacterial inoculum having 103 and 106 CFU/mL respectively. The rabbits of Group C served as control group. Rectal temperature of each rabbit was recorded daily. On postmortem, CHAPTER 6 SUMMARY Summary 67 gross and microscopic lesions were recorded. The results showed that rabbits of control group not showed any gross or microscopic change. There was significant increase in rectal temperature of infected rabbits as compared to uninfected rabbits. The gross lesions were specific for the organism which was prominently observed in lungs of rabbits. The microscopic lesions revealed that there was severe consolidation, congestion and fibrin exudation in lungs of rabbits of group A which were given less number of organism and they developed clear signs of disease. The rabbits of Group B showed less prominent signs compared to group A due to early death of rabbits. There were multiple hemorrhages, of varying sizes and hyalinization of myocardial cells in infected rabbits. The severity of changes was significantly more different in Group A, as compared to Group B. It can be deduced by this study that the rabbit can be used as a model for further studies exploring the pathogenesis of the disease as the lesions resemble to shipping fever caused by M. hemolytica in ruminants. The lesions, which developed, could be descending infection resulting in typical lesions of bronchopneumonia or lobular pneumonia. Availability: Items available for loan: UVAS Library [Call number: 2517-T] (1).

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